Tag: Environment

  • Cellular Respiration

    What is Cellular Respiration?

    Some organisms, such as plants, can trap the energy in sunlight through photosynthesis (see Photosynthesis) and store it in the chemical bonds of carbohydrate molecules. The principal carbohydrate formed through photosynthesis is glucose. Other types of organisms, such as animals, fungi, many protozoa, and a large portion of bacteria, are unable to perform this process. Therefore, these organisms must rely on the carbohydrates formed in plants to obtain the energy necessary for their metabolic processes.

    Animals and other organisms obtain the energy available in carbohydrates through the process of cellular respiration. Cells take the carbohydrates into their cytoplasm, and through a complex series of metabolic processes, they break down the carbohydrates and release the energy. The energy is generally not needed immediately; rather, it is used to combine adenosine diphosphate (ADP) with phosphate ions to form adenosine triphosphate (ATP) molecules. The ATP can then be used for processes in the cells that require energy, much as a battery powers a mechanical device.

    During the process of cellular respiration, carbon dioxide is given off. This carbon dioxide can be used by plant cells during photosynthesis to form new carbohydrates. Also in the process of cellular respiration, oxygen gas is required to serve as an acceptor of electrons. This oxygen is identical to the oxygen gas given off during photosynthesis. Thus, there is an interrelationship between the processes of photosynthesis and cellular respiration, namely the entrapment of energy available in sunlight and the provision of the energy for cellular processes in the form of ATP.

    The overall mechanism of cellular respiration involves four processes: glycolysis, in which glucose molecules are broken down to form pyruvic acid molecules; the Krebs cycle, in which pyruvic acid is further broken down and the energy in its molecule is used to form high-energy compounds, such as nicotinamide adenine dinucleotide (NADH); the electron transport system, in which electrons are transported along a series of coenzymes and cytochromes and the energy in the electrons is released; and chemiosmosis, in which the energy given off by electrons pumps protons across a membrane and provides the energy for ATP synthesis. The general chemical equation for cellular respiration is:

    C6H12O6 + 6 O2 → 6 H2O + 6CO2 + energy

    Figure 1. provides an overview of cellular respiration. Glucose is converted to pyruvic acid in the cytoplasm, which is then used to produce acetyl CoA in the mitochondrion. Finally, the Krebs cycle proceeds in the mitochondrion. Electron transport and chemiosmosis result in energy release; ATP synthesis also occurs in the mitochondrion.

    Glycolysis

    Glycolysis is the process in which one glucose molecule is broken down to form two molecules of pyruvic acid (also called pyruvate). The glycolysis process is a multi-step metabolic pathway that occurs in the cytoplasm of animal cells, plant cells, and the cells of microorganisms. At least six enzymes operate in the metabolic pathway.

    In the first and third steps of the pathway, ATP energizes the molecules. Thus, two ATP molecules must be expended in the process. Further along in the process, the six-carbon glucose molecule converts into intermediary compounds and is then split into two three-carbon compounds. The latter undergo additional conversions and eventually form pyruvic acid at the conclusion of the process.

    During the latter stages of glycolysis, four ATP molecules are synthesized using the energy given off during the chemical reactions. Thus, four ATP molecules are synthesized and two ATP molecules are used during glycolysis, for a net gain of two ATP molecules.

    An overview of cellular respiration

    Figure 1. An overview of cellular respiration.

    Another reaction during glycolysis yields enough energy to convert NAD to NADH (plus a hydrogen ion). The reduced coenzyme (NADH) will later be used in the electron transport system, and its energy will be released. During glycolysis, two NADH molecules are produced.

    Because glycolysis does not require oxygen, the process is considered to be anaerobic. For certain anaerobic organisms, such as some bacteria and fermentation yeasts, glycolysis is the sole source of energy.

    Glycolysis is a somewhat inefficient process because much of the cellular energy remains in the two molecules of pyruvic acid that are created. Interestingly, this process is somewhat similar to a reversal of photosynthesis (see Photosynthesis).

    Krebs Cycle

    Following glycolysis, the mechanism of cellular respiration involves another multi-step process—the Krebs cycle, which is also called the citric acid cycle or the tricarboxylic acid cycle. The Krebs cycle uses the two molecules of pyruvic acid formed in glycolysis and yields high-energy molecules of NADH and Flavin adenine dinucleotide (FADH2), as well as some ATP.

    Krebs Cycle
    Krebs Cycle

    The Krebs cycle occurs in the mitochondrion of a cell (see Figure 6). This sausage-shaped organelle possesses inner and outer membranes and, therefore, inner and outer compartments. The inner membrane is folded over itself many times; the folds are called cristae. They are somewhat similar to the thylakoid membranes in chloroplasts (see Photosynthesis). Located along the cristae are the important enzymes necessary for the proton pump and for ATP production.

    Prior to entering the Krebs cycle, the pyruvic acid molecules are altered. Each three-carbon pyruvic acid molecule undergoes conversion to a substance called acetyl-coenzyme A, or Acetyl-CoA. During the process, the pyruvic acid molecule is broken down by an enzyme, one carbon atom is released in the form of carbon dioxide, and the remaining two carbon atoms are combined with a coenzyme called coenzyme A. This combination forms Acetyl-CoA. In the process, electrons and a hydrogen ion are transferred to NAD to form high-energy NADH.

    Acetyl-CoA enters the Krebs cycle by combining with a four-carbon acid called oxaloacetic acid. The combination forms the six-carbon acid called citric acid. Citric acid undergoes a series of enzyme-catalyzed conversions. The conversions, which involve up to ten chemical reactions, are all brought about by enzymes. In many of the steps, high-energy electrons are released to NAD. The NAD molecule also acquires a hydrogen ion and becomes NADH. In one of the steps, FAD serves as the electron acceptor, and it acquires two hydrogen ions to become FADH2. Also, in one of the reactions, enough energy is released to synthesize a molecule of ATP. Because for each glucose molecule there are two pyruvic acid molecules entering the system, two ATP molecules are formed.

    Also during the Krebs cycle, the two carbon atoms of Acetyl-CoA are released, and each forms a carbon dioxide molecule. Thus, for each Acetyl-CoA entering the cycle, two carbon dioxide molecules are formed. Two Acetyl-CoA molecules enter the cycle, and each has two carbon atoms, so four carbon dioxide molecules will form. Add these four molecules to the two carbon dioxide molecules formed in the conversion of pyruvic acid to Acetyl-CoA, and it adds up to six carbon dioxide molecules. These six CO2 molecules are given off as waste gas in the Krebs cycle. They represent the six carbons of glucose that originally entered the process of glycolysis.

    At the end of the Krebs cycle, the final product is oxaloacetic acid. This is identical to the oxaloacetic acid that begins the cycle. Now the molecule is ready to accept another Acetyl-CoA molecule to begin another turn of the cycle. All told, the Krebs cycle forms (per two molecules of pyruvic acid) two ATP molecules, ten NADH molecules, and two FADH2 molecules. The NADH and the FADH2 will be used in the electron transport system.

    Electron Transport System

    The electron transport system occurs in the cristae of the mitochondria, where a series of cytochromes (enzymes) and coenzymes exist. These cytochromes and coenzymes act as carrier molecules and transfer molecules. They accept high-energy electrons and pass the electrons to the next molecule in the system. At key proton-pumping sites, the energy of the electrons transports protons across the membrane into the outer compartment of the mitochondrion.

    Each NADH molecule is highly energetic, which accounts for the transfer of six protons into the outer compartment of the mitochondrion. Each FADH2 molecule accounts for the transfer of four protons. The flow of electrons is similar to that taking place in photosynthesis. Electrons pass from NAD to FAD, to other cytochromes and coenzymes, and eventually they lose much of their energy. In cellular respiration, the final electron acceptor is an oxygen atom. In their energy-depleted condition, the electrons unite with an oxygen atom. The electron-oxygen combination then reacts with two hydrogen ions (protons) to form a water molecule (H2O).

    The role of oxygen in cellular respiration is substantial. As a final electron acceptor, it is responsible for removing electrons from the electron transport system. If oxygen were not available, electrons could not be passed among the coenzymes, the energy in electrons could not be released, the proton pump could not be established, and ATP could not be produced. In humans, breathing is the essential process that brings oxygen into the body for delivery to the cells to participate in cellular respiration.

    Chemiosmosis

    The actual production of ATP in cellular respiration takes place through the process of chemiosmosis (see Cells and Energy). Chemiosmosis involves the pumping of protons through special channels in the membranes of mitochondria from the inner to the outer compartment. The pumping establishes a proton (H+) gradient. After the gradient is established, protons diffuse down the gradient through a transport protein called ATP synthase. The flow of hydrogens catalyzes the pairing of a phosphate with ADP, forming ATP.

    The energy production of cellular respiration is substantial. Most biochemists agree that 36 molecules of ATP can be produced for each glucose molecule during cellular respiration as a result of the Krebs cycle reactions, the electron transport system, and chemiosmosis. Also, two ATP molecules are produced through glycolysis, so the net yield is 38 molecules of ATP. These ATP molecules may then be used in the cell for its needs. However, the ATP molecules cannot be stored for long periods of time, so cellular respiration must constantly continue in order to regenerate the ATP molecules as they are used. Each ATP molecule is capable of releasing 7.3 kilocalories of energy per mole.

    Fermentation

    Fermentation is an anaerobic process in which energy can be released from glucose even though oxygen is not available. Fermentation occurs in yeast cells, and a form of fermentation takes place in bacteria and in the muscle cells of animals.

    In yeast cells (the yeast used for baking bread and producing alcoholic beverages), glucose can be metabolized through cellular respiration as in other cells. When oxygen is lacking, however, glucose is still metabolized to pyruvic acid via glycolysis. The pyruvic acid is converted first to acetaldehyde and then to ethyl alcohol. The net gain of ATP to the yeast cell is two molecules—the two molecules of ATP normally produced in glycolysis.

    Yeasts are able to participate in fermentation because they have the necessary enzyme to convert pyruvic acid to ethyl alcohol. This process is essential because it removes electrons and hydrogen ions from NADH during glycolysis. The effect is to free the NAD so it can participate in future reactions of glycolysis. The net gain to the yeast cell of two ATP molecules permits it to remain alive for some time. However, when the percentage of ethyl alcohol reaches approximately 15 percent, the alcohol kills the yeast cells.

    Yeast is used in both bread and alcohol production. Alcohol fermentation is the process that yields beer, wine, and other spirits. The carbon dioxide given off during fermentation supplements the carbon dioxide given off during the Krebs cycle and causes bread to rise.

    In muscle cells, another form of fermentation takes place. When muscle cells contract too frequently (as in strenuous exercise), they rapidly use up their oxygen supply. As a result, the electron transport system and Krebs cycle slow considerably, and ATP production is slowed. However, muscle cells have the ability to produce a small amount of ATP through glycolysis in the absence of oxygen. The muscle cells convert glucose to pyruvic acid. An enzyme in the muscle cells then converts the pyruvic acid to lactic acid. As in the yeast, this reaction frees up the NAD while providing the cells with two ATP molecules from glycolysis. Eventually, however, the lactic acid buildup causes intense fatigue, and the muscle stops contracting.

  • Photosynthesis

    What is Photosynthesis?

    A great variety of living things on Earth, including all green plants, synthesize their foods from simple molecules, such as carbon dioxide and water. For this process, the organisms require energy, and that energy is derived from sunlight.

    Figure 1. shows the energy relationships in living cells. Light energy is captured in the chloroplast of plant cells and used to synthesize glucose molecules, shown as C6H12O6. In the process, oxygen (O2) is released as a waste product. The glucose and oxygen are then used in the mitochondrion of the plant cell, and the energy is released and used to fuel the synthesis of ATP from ADP and P. In the reaction, CO2 and water are released in the mitochondrion to be reused in photosynthesis in the chloroplast.

    Energy relationships in living cells Cycles
    Energy relationships in living cells Cycles

    Energy relationships in living cells

    Figure 1. Energy relationships in living cells.

    The process of utilizing energy to synthesize carbohydrate molecules is called photosynthesis. Photosynthesis is actually two separate processes. in the first process, energy-rich electrons flow through a series of coenzymes and other molecules. This electron energy is trapped. During the trapping process, adenosine triphosphate (ATP) molecules and molecules of nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) are formed. Both ATP and NADPH are rich in energy. These molecules are used in the second process, where carbon dioxide molecules are bound into carbohydrates too form organic substances such as glucose.

    Chloroplast

    The organelle in which photosynthesis occurs (in the leaves and green stems of plants, for example) is called the chloroplast. Chloroplasts are relatively large organelles, containing a watery, protein-rich fluid called stroma. The stroma contains many small structures composed of membranes that resemble stacks of coins. Each stack is a granum (the plural form is grana). Each membrane in the stack is a thylakoid. Within the thylakoid membranes of the granum, many of the reactions of photosynthesis take place. The thylakoids are somewhat similar to the cristae of mitochondria (see Cellular Respiration).

    Photosystems

    Pigment molecules organized into photosystems capture sunlight in the chloroplast. Photosystems are clusters of light-absorbing pigments with some associated molecules—proton (hydrogen ion) pumps, enzymes, coenzymes, and cytochromes (see Cells and Energy). Each photosystem contains about 200 molecules of a green pigment called chlorophyll and about 50 molecules of another family of pigments called carotenoids. In the reaction center of the photosystem, the energy of sunlight is converted to chemical energy. The center is sometimes called a light-harvesting antenna.

    There are two photosystems within the thylakoid membranes, designated photosystem I and photosystem II. The reaction centers of these photosystems are P700 and P680, respectively. The energy captured in these reaction centers drives chemiosmosis, and the energy of chemiosmosis stimulates ATP production in the chloroplasts.

    Process of Photosynthesis

    The process of photosynthesis is conveniently divided into two parts: the energy-fixing reaction (also called the light reaction) and the carbon-fixing reaction (also called the light-independent reaction or the dark reaction).

    Energy-fixing reaction

    The energy-fixing reaction of photosynthesis begins when light is absorbed in photosystem II in the thylakoid membranes. The energy of the sunlight, captured in the P680 reaction center, causes the electrons from P680’s chlorophyll to move to a higher, unstable energy level. These electrons pass through a series of cytochromes in the nearby electron-transport system.

    After passing through the electron transport system, the energy-rich electrons eventually enter Photosystem-I. Some of the energy of the electron is used to pump protons across the thylakoid membrane, and this pumping sets up the potential for chemiosmosis.

    The spent electrons from P680 enter the P700 reaction center in photosystem I. Sunlight activates the electrons, which receive a second boost out of the chlorophyll molecules. There they reach a high energy level. The electrons progress through a second electron transport system, but this time there is no proton pumping. Rather, the energy reduces NADP. This reduction occurs as two electrons join NADP and energize the molecule. Because NADP acquires two negatively charged electrons, it attracts two positively charged protons to balance the charges. Consequently, the NADP molecule is reduced to NADPH, a molecule that contains much energy.

    Because electrons have flowed out of the P680 reaction center, the chlorophyll molecules are left without a certain number of electrons. Electrons secured from water molecules replace these electrons. Each split water molecule releases two electrons that enter the chlorophyll molecules to replace those lost. The split water molecules also release two protons that enter the cytoplasm near the thylakoid and are available to increase the chemiosmotic gradient.

    The third product of the split water molecules is oxygen. Two oxygen atoms combine with one another to form molecular oxygen (O2), which is given off as the by-product of photosynthesis; it fills the atmosphere and is used by all oxygen-requiring organisms, including plant and animal cells.

    Described above are the noncyclic energy-fixing reactions (see Figure 2). Certain plants and autotrophic prokaryotes are also known to participate in cyclic energy-fixing reactions. These reactions involve only photosystem I and the P700 reaction center. Excited electrons leave the reaction center, pass through coenzymes of the electron transport system, and follow a special pathway back to P700. Each electron powers the proton pump and encourages the transport of a proton across the thylakoid membrane. This process enriches the proton gradient and eventually leads to the generation of ATP.

    The energy-fixing reactions of photosynthesis

    Figure 2. The energy-fixing reactions of photosynthesis.

    ATP production in the energy-fixing reactions of photosynthesis occurs by the process of chemiosmosis (explained in Cells and Energy). Essentially, this process consists of a rush of protons across a membrane (the thylakoid membrane, in this case), accompanied by the synthesis of ATP molecules. Biochemists have calculated that the proton concentration on one side of the thylakoid is 10,000 times that of the opposite side of the membrane.

    In photosynthesis, the protons pass back across the membranes through channels lying alongside sites where enzymes are located. As the protons pass through the channels, the energy of the protons is released to form high-energy ATP bonds. ATP is formed in the energy-fixing reactions along with the NADPH formed in the main reactions. Both ATP and NADPH provide the energy necessary for the synthesis of carbohydrates that occurs in the second major set of events in photosynthesis.

    Carbon-fixing reaction

    Glucose and other carbohydrates are synthesized in the carbon-fixing reaction of photosynthesis, often called the Calvin cycle after Melvin Calvin, who performed much of the biochemical research (see Figure 3). This phase of photosynthesis occurs in the stroma of the plant cell.

    A carbon-fixing reaction or the Calvin cycle
    A carbon-fixing reaction or the Calvin cycle

    Figure 3. A carbon-fixing reaction, also called the Calvin cycle.

    In the carbon-fixing reaction, an essential material is carbon dioxide, which is obtained from the atmosphere. The carbon dioxide is attached to a five-carbon compound called ribulose bisphosphate. Ribulose bisphosphate carboxylase catalyzes this reaction.

    After carbon dioxide has been joined to ribulose bisphosphate, a six-carbon product forms, which immediately breaks into two three-carbon molecules called phosphoglycerate. Each phosphoglycerate molecule converts to another organic compound, but only in the presence of ATP. The ATP used is the ATP synthesized in the energy-fixing reaction. The organic compound formed converts to still another organic compound using the energy present in NADPH. Again, the energy-fixing reaction provides the essential energy. Each of the organic compounds that results consists of three carbon atoms. Eventually, the compounds interact with one another and join to form a single molecule of six-carbon glucose. This process also generates additional molecules of ribulose bisphosphate to participate in further carbon-fixing reactions.

    Glucose can be stored in plants in several ways. In some plants, the glucose molecules are joined to one another to form starch molecules. Potato plants, for example, store starch in tubers (underground stems). In some plants, glucose converts to fructose (fruit sugar), and the energy is stored in this form. In still other plants, fructose combines with glucose to form sucrose, commonly known as table sugar. The energy is stored in carbohydrates in this form. Plant cells obtain energy for their activities from these molecules. Animals use the same forms of glucose by consuming plants and delivering the molecules to their cells.

    All living things on Earth depend in some way on photosynthesis. It is the main mechanism for bringing the energy of sunlight into living systems and making that energy available for the chemical reactions taking place in cells.

  • Cells and Energy

    What are Cells and Energy?

    The Laws of Thermodynamics; Life can exist only where molecules and cells remain organized. All cells need energys to maintain organization. Physicists define energy as the ability to do work; in this case, the work is the continuation of life itself.

    Energy has been expressed in terms of reliable observations known as the laws of thermodynamics. There are two such laws. The first law of thermodynamics states that energy can neither be created nor destroyed. This law implies that the total amount of energy in a closed system (for example, the universe) remains constant. Energys neither enters nor leaves a closed system.

    Cells and Energy 1

    Within a closed system, energy can change, however. For instance, the chemical energy in gasoline is released when the fuel combines with oxygen and a spark ignites the mixture within a car’s engine. The gasoline’s chemical energy is changed into heat energy, sound energy, and the energy of motion.

    The second law of thermodynamics states that the amount of available energy in a closed system is decreasing constantly. Energy becomes unavailable for use by living things because of entropy, which is the degree of disorder or randomness of a system. The entropy of any closed system is constantly increasing. In essence, any closed system tends toward disorganization.

    Unfortunately, the transfers of energy in living systems are never completely efficient. Every body movement, every thought, and every chemical reaction in the cells involves a shift of energy and a measurable decrease of energy available to do work in the process. For this reason, considerably more energy must be taken into the system than is necessary to carry out the actions of life.

    Chemical Reactions

    Most chemical compounds do not combine with one another automatically, nor do chemical compounds break apart automatically. The great majority of the chemical reactions that occur within living things must be energized. This means that the atoms of a molecule must be separated by energy put into the system. The energy forces apart the atoms in the molecules and allows the reaction to take place.

    To initiate a chemical reaction, a type of “spark,” referred to as the energy of activation, is needed. For example, hydrogen and oxygen can combine to form water at room temperature, but the reaction requires activation energy.

    Any chemical reaction in which energy is released is called an exergonic reaction. In an exergonic chemical reaction, the products end up with less energy than the reactants. Other chemical reactions are endergonic reactions. In endergonic reactions, energy is obtained and trapped from the environment. The products of endergonic reactions have more energy than the reactants taking part in the chemical reaction. For example, plants carry out the process of photosynthesis, in which they trap energy from the sun to form carbohydrates (see Photosynthesis).

    The activation energy needed to spark an exergonic or endergonic reaction can be heat energy or chemical energy. Reactions that require activation energy can also proceed in the presence of biological catalysts. Catalysts are substances that speed up chemical reactions but remain unchanged themselves. Catalysts work by lowering the required amount of activation energy for the chemical reaction. For example, hydrogen and oxygen combine with one another in the presence of platinum. In this case, platinum is the catalyst. In biological systems, the most common catalysts are protein molecules called enzymes. Enzymes are absolutely essential if chemical reactions are to occur in cells.

    Enzymes

    The chemical reactions in all cells of living things operate in the presence of biological catalysts called enzymes. Because a particular enzyme catalyzes only one reaction, there are thousands of different enzymes in a cell catalyzing thousands of different chemical reactions. The substance changed or acted on by an enzyme is its substrate. The products of a chemical reaction catalyzed by an enzyme are end products.

    All enzymes are composed of proteins. (Proteins are chains of amino acids; see The Chemical Basis of Life.) When an enzyme functions, a key portion of the enzyme, called the active site, interacts with the substrate. The active site closely matches the molecular configuration of the substrate. After this interaction has taken place, a change in shape in the active site places a physical stress on the substrate. This physical stress aids the alteration of the substrate and produces the end products. During the time the active site is associated with the substrate, the combination is referred to as the enzyme-substrate complex. After the enzyme has performed its work, the product or products are released from the enzyme’s active site. The enzyme is then free to function in another chemical reaction.

    Enzyme-catalyzed reactions occur extremely fast. They happen about a million times faster than uncatalyzed reactions. With some exceptions, the names of enzymes end in “–ase.” For example, the enzyme that breaks down hydrogen peroxide to water and hydrogen is catalase. Other enzymes include amylase, hydrolase, peptidase, and kinase.

    The rate of an enzyme-catalyzed reaction depends on a number of factors, such as the concentration of the substrate, the acidity and temperature of the environment, and the presence of other chemicals. At higher temperatures, enzyme reactions occur more rapidly, but only up to a point. Because enzymes are proteins, excessive amounts of heat can change their structures, rendering them inactive. An enzyme altered by heat is said to be denatured.

    Enzymes work together in metabolic pathways. A metabolic pathway is a sequence of chemical reactions occurring in a cell. A single enzyme-catalyzed reaction may be one of multiple reactions in a metabolic pathway. Metabolic pathways may be of two general types: catabolic and anabolic. Catabolic pathways involve the breakdown or digestion of large, complex molecules. The general term for this process is catabolism. Anabolic pathways involve the synthesis of large molecules, generally by joining smaller molecules together. The general term for this process is anabolism.

    Many enzymes are assisted by chemical substances called cofactors. Cofactors may be ions or molecules associated with an enzyme and are required in order for a chemical reaction to take place. Ions that might operate as cofactors include those of iron, manganese, and zinc. Organic molecules acting as cofactors are referred to as coenzymes.

    Adenosine Triphosphate (ATP)

    Adenosine Triphosphate (ATP)

    The chemical substance that serves as the currency of energy in a cell is adenosine triphosphate (ATP). ATP is referred to as currency because it can be “spent” in order to make chemical reactions occur. The more energy required for a chemical reaction, the more ATP molecules must be spent.

    Virtually all forms of life use ATP, a nearly universal molecule of energy transfer. The energy released during catabolic reactions is stored in ATP molecules. In addition, the energy trapped in anabolic reactions (such as photosynthesis) is trapped in ATP molecules.

    An ATP molecule consists of three parts. One part is a double ring of carbon and nitrogen atoms called adenine. Attached to the adenine molecule is a small five-carbon carbohydrate called ribose. Attached to the ribose molecule are three phosphate units linked together by covalent bonds.

    Adenosine Triphosphate Structure
    Adenosine Triphosphate Structure

    The covalent bonds that unite the phosphate units in ATP are high-energy bonds. When an ATP molecule is broken down by an enzyme, the third (terminal) phosphate unit is released as a phosphate group, which is an ion. When this happens, approximately 7.3 kilocalories of energy are released. (A kilocalorie equals 1,000 calories.) This energy is made available to do the work of the cell.

    The adenosine triphosphatase enzyme accomplishes the breakdown of an ATP molecule. The products of ATP breakdown are adenosine diphosphate (ADP) and a phosphate ion. Adenosine diphosphate and the phosphate ion can be reconstituted to form ATP, much like a battery can be recharged. To accomplish this, synthesis energy must be available. This energy can be made available in the cell through two extremely important processes: photosynthesis.

    ATP Production

    ATP is generated from ADP and phosphate ions by a complex set of processes occurring in the cell. These processes depend on the activities of a special group of coenzymes. Three important coenzymes are nicotinamide adenine dinucleotide (NAD), nicotinamide adenine dinucleotide phosphate (NADP), and Flavin adenine dinucleotide (FAD).

    NAD and NADP are structurally similar to ATP. Both molecules have a nitrogen-containing ring called nicotinic acid, which is the chemically active part of the coenzymes. In FAD, the chemically active portion is the Flavin group. The vitamin riboflavin is used in the body to produce this Flavin group.

    All coenzymes perform essentially the same work. During the chemical reactions of metabolism, coenzymes accept electrons and pass them on to other coenzymes or other molecules. The removal of electrons or protons from a coenzyme is oxidation. The addition of electrons to a molecule is reduction. Therefore, the chemical reactions performed by coenzymes are called oxidation-reduction reactions.

    The oxidation-reduction reactions performed by the coenzymes and other molecules are essential to the energy metabolism of the cell. Other molecules participating in this energy reaction are called cytochromes. Together with the coenzymes, cytochromes accept and release electrons in a system called the electron transport system. The passage of energy-rich electrons among cytochromes and coenzymes drains the energy from the electrons to form ATP from ADP and phosphate ions.

    The actual formation of ATP molecules requires a complex process called chemiosmosis. Chemiosmosis involves the creation of a steep proton (hydrogen ion) gradient. This gradient occurs between the membrane-bound compartments of the mitochondria of all cells and the chloroplasts of plant cells. A gradient is formed when large numbers of protons (hydrogen ions) are pumped into the membrane-bound compartments of the mitochondria. The protons build up dramatically within the compartment, finally reaching an enormous number. The energy released from the electrons during the electron transport system pumps the protons.

    After large numbers of protons have gathered within the compartments of mitochondria and chloroplasts, they suddenly reverse their directions and escape back across the membranes and out of the compartments. The escaping protons release their energy in this motion. This energy is used by enzymes to unite ADP with phosphate ions to form ATP. The energy is trapped in the high-energy bond of ATP by this process, and the ATP molecules are made available to perform cell work. The movement of protons is chemiosmosis because it is a movement of chemicals (in this case, protons) across a semipermeable membrane. Because chemiosmosis occurs in mitochondria and chloroplasts, these organelles play an essential role in the cell’s energy metabolism. Photosynthesis explains how energy is trapped in the chloroplasts in plants, while Cellular Respiration explains how energy is released in the mitochondria of plant and animal cells.

  • What is The Chemical Basis of Life?

    What is The Chemical Basis of Life?

    Understanding of Chemical; A chemical substance is a form of matter that has the constant chemical composition and characteristic properties. It cannot be separated into components by physical separation methods, i.e., without breaking chemical bonds. Chemical substances can be chemical elements, chemical compounds, ions or alloys.

    Chemical substances are often called ‘pure’ to set them apart from mixtures. A common example of a chemical substance is pure water; it has the same properties and the same ratio of hydrogen to oxygen whether it is isolated from a river or made in a laboratory. Other chemical substances commonly encountered in pure form are the diamond (carbon), gold, table salt (sodium chloride) and refined sugar (sucrose). However, in practice, no substance is entirely pure, and chemical purity is specified according to the intended use of the chemical.

    Chemical substances exist as solids, liquids, gases, or plasma, and may change between these phases of matter with changes in temperature or pressure. Chemical substances may be combined or converted to others by means of chemical reactions. Now you will understanding of The Chemical Basis of Life.

    Acids and Bases

    Acids are chemical compounds that release hydrogen ions (H+) when placed in water. For example, when hydrogen chloride is placed in water, it releases its hydrogen ions and the solution becomes hydrochloric acid.

    Bases are chemical compounds that attract hydrogen atoms when they are placed in water. An example of a base is sodium hydroxide (NaOH). When this substance is placed in water, it attracts hydrogen ions, and a basic (or alkaline) solution results as hydroxyl (–OH) ions accumulate.

    Molecule

    Most of the compounds of interest to biologists are composed of units called molecules. A molecule is a precise arrangement of atoms held together by chemical bonds, and a compound is a molecule that contains atoms of more than one element. A molecule may be composed of two or more atoms of the same element, as in oxygen gas (O2), or it may be composed of atoms from different elements. The arrangements of the atoms in a molecule account for the properties of a compound. The molecular weight is equal to the atomic weights of the atoms in the molecule.

    The atoms in molecules may be joined to one another by various linkages called bonds. One example of a bond is an ionic bond, which is formed when the electrons of one atom transfer to a second atom. This creates electrically charged atoms called ions. The electrical charges cause the ions to be attracted to one another, and the attraction forms the ionic bond.

    A second type of linkage is a covalent bond. A covalent bond forms when two atoms share one or more electrons with one another. For example, as shown in Figure 1, oxygen shares its electrons with two hydrogen atoms, and the resulting molecule is water (H2O). Nitrogen shares its electrons with three hydrogen atoms, and the resulting molecule is ammonia (NH3). If one pair of electrons is shared, the bond is a single bond; if two pairs are shared, it is a double bond.

    Formation of a covalent bond in water and ammonia molecules

    Figure 1. Formation of a covalent bond in water and ammonia molecules. In each molecule, the second shell fills with eight electrons.

    Organic Compound: The chemical compounds of living things are known as organic compounds because of their association with organisms and because they are carbon-containing compounds. Organic compounds, which are the compounds associated with life processes, are the subject matter of organic chemistry. Among the numerous types of organic compounds, four major categories are found in all living things: carbohydrates, lipids, proteins, and nucleic acids.

    Carbohydrates

    Almost all organisms use carbohydrates as sources of energy. In addition, some carbohydrates serve as structural materials. Carbohydrates are molecules composed of carbon, hydrogen, and oxygen; the ratio of hydrogen atoms to oxygen and carbon atoms is 2:1.

    Simple carbohydrates, commonly referred to as sugars, can be monosaccharides if they are composed of single molecules, or disaccharides if they are composed of two molecules. The most important monosaccharide is glucose, a carbohydrate with the molecular formula C6H12O6. Glucose is the basic form of fuel in living things. In multicellular organisms, it is soluble and is transported by body fluids to all cells, where it is metabolized to release its energy. Glucose is the starting material for cellular respiration, and it is the main product of photosynthesis

    Three important disaccharides are also found in living things: maltose, sucrose, and lactose. Maltose is a combination of two glucose units covalently linked. The table sugar sucrose is formed by linking glucose to another monosaccharide called fructose. (Figure 2 shows that in the synthesis of sucrose, a water molecule is produced. The process is therefore called a dehydration reaction. The reversal of the process is hydrolysis, a process in which the molecule is split and water is added.) Lactose is composed of glucose and galactose units.

    Glucose and fructose molecules combine to form the disaccharide sucrose

    Figure 2. Glucose and fructose molecules combine to form the disaccharide sucrose.

    Complex carbohydrates are known as polysaccharides. Polysaccharides are formed by linking innumerable monosaccharides. Among the most important polysaccharides is starch, which is composed of hundreds or thousands of glucose units linked to one another. Starch serves as a storage form for carbohydrates. Much of the world’s human population satisfies its energy needs with starch in the form of rice, wheat, corn, and potatoes.

    Two other important polysaccharides are glycogen and cellulose. Glycogen is also composed of thousands of glucose units, but the units are bonded in a different pattern than in starch. Glycogen is the form in which glucose is stored in the human liver. Cellulose is used primarily as a structural carbohydrate. It is also composed of glucose units, but the units cannot be released from one another except by a few species of organisms. Wood is composed chiefly of cellulose, as are plant cell walls. Cotton fabric and paper are commercial cellulose products.

    Lipids

    Lipids are organic molecules composed of carbon, hydrogen, and oxygen atoms. The ratio of hydrogen atoms to oxygen atoms is much higher in lipids than in carbohydrates. Lipids include steroids (the material of which many hormones are composed), waxes, and fats.

    Fat molecules are composed of a glycerol molecule and one, two, or three molecules of fatty acids (see Figure 3). A glycerol molecule contains three hydroxyl (–OH) groups. A fatty acid is a long chain of carbon atoms (from 4 to 24) with a carboxyl (–COOH) group at one end. The fatty acids in a fat may all be alike or they may all be different. They are bound to the glycerol molecule by a process that involves the removal of water.

    Certain fatty acids have one or more double bonds in their molecules. Fats that include these molecules are unsaturated fats. Other fatty acids have no double bonds. Fats that include these fatty acids are saturated fats. In most human health situations, the consumption of unsaturated fats is preferred to the consumption of saturated fats.

    Fats stored in cells usually form clear oil droplets called globules because fats do not dissolve in water. Plants often store fats in their seeds, and animals store fats in large, clear globules in the cells of adipose tissue. The fats in adipose tissue contain much concentrated energy. Hence, they serve as a reserve energy supply to the organism. The enzyme lipase breaks down fats into fatty acids and glycerol in the human digestive system.

    A fat molecule is constructed by combining a glycerol molecule with three fatty acid molecules

    Figure 3. A fat molecule is constructed by combining a glycerol molecule with three fatty acid molecules. (Two saturated fatty acids and one unsaturated fatty acid are shown for comparison.) The constructed molecule is at the bottom.

    Protein

    Proteins, among the most complex of all organic compounds, are composed of amino acids (see Figure 4), which contain carbon, hydrogen, oxygen, and nitrogen atoms. Certain amino acids also have sulfur atoms, phosphorus, or other trace elements such as iron or copper.

    The structure and chemistry of amino acids

    Figure 4. The structure and chemistry of amino acids. When two amino acids are joined in a dipeptide, the –OH of one amino acid is removed, and the –H of the second is removed. So, water is removed. A dipeptide bond (right) forms to join the amino acids together

    Many proteins are immense and extremely complex. However, all proteins are composed of long chains of relatively simple amino acids. There are 20 kinds of amino acids. Each amino acid (see the left illustration in Figure 4) has an amino (–NH2) group, a carboxyl (–COOH) group, and a group of atoms called an –R group (where R stands for radical). The amino acids differ depending on the nature of the –R group, as shown in the middle illustration of Figure 4. Examples of amino acids are alanine, valine, glutamic acid, tryptophan, tyrosine, and histidine.

    The removal of water molecules links amino acids to form a protein. The process is called dehydration synthesis, and a by-product of the synthesis is water. The links forged between the amino acids are peptide bonds, and small proteins are often called peptides.

    All living things depend on proteins for their existence. Proteins are the major molecules from which living things are constructed. Certain proteins are dissolved or suspended in the watery substance of the cells, while others are incorporated into various structures of the cells. Proteins are also found as supporting and strengthening materials in tissues outside of cells. Bone, cartilage, tendons, and ligaments are all composed of proteins.

    One essential function of proteins is as an enzyme. Enzymes catalyze the chemical reactions that take place within cells. They are not used up in a reaction; rather, they remain available to catalyze succeeding reactions.

    Every species manufactures proteins unique to that species. The information for synthesizing the unique proteins is located in the nucleus of the cell. The so-called genetic code specifies the amino acid sequence in proteins. Hence, the genetic code regulates the chemistry taking place within a cell. Proteins also can serve as a reserve source of energy for the cell. When the amino group is removed from an amino acid, the resulting compound is energy-rich.

    Nucleic acids: Like proteins, nucleic acids are very large molecules. The nucleic acids are composed of smaller units called nucleotides. Each nucleotide contains a carbohydrate molecule (sugar), a phosphate group, and a nitrogen-containing molecule that, because of its properties, is a nitrogenous base.

    Living organisms have two important nucleic acids. One type is deoxyribonucleic acid, or DNA. The other is ribonucleic acid, or RNA. DNA is found primarily in the nucleus of the cell, while RNA is found in both the nucleus and the cytoplasm, a semiliquid substance that composes the volume of the cell.

    DNA and RNA differ from one another in their components. DNA contains the carbohydrate deoxyribose, while RNA has ribose. In addition, DNA contains the base thymine, while RNA has uracil.

    Elements and Atoms

    For many centuries, biology was the study of the natural world. Biologists searched for unidentified plants and animals, classified them, and studied their anatomy and how they acted in nature. Then in the 1700s, scientists discovered the chemical and physical bases of living things. They soon realized that the chemical organization of all living things is remarkably similar.

    Elements: All living things on Earth are composed of fundamental building blocks of matter called elements. More than 100 elements are known to exist, including those that are man-made. An element is a substance that cannot be chemically decomposed. Oxygen, iron, calcium, sodium, hydrogen, carbon, and nitrogen are examples of elements.

    Atoms: Each element is composed of one particular kind of atom. An atom is the smallest part of an element that can enter into combinations with atoms of other elements.

    Atoms consist of positively charged particles called protons surrounded by negatively charged particles called electrons. A third type of particle, a neutron, has no electrical charge; it has the same weight as a proton. Protons and neutrons adhere tightly to form the dense, positively charged nucleus of the atom. Electrons spin around the nucleus.

    The electron arrangement in an atom plays an essential role in the chemistry of the atom. Atoms are most stable when their outer shell of electrons has a full quota. The first electron shell has a maximum of two electrons. The second and all other outer shells have a maximum of eight electrons. Atoms tend to gain or lose electrons until their outer shells have a stable arrangement. The gaining or losing of electrons, or the sharing of electrons, contributes to the chemical reactions in which an atom participates.

  • What is Cells Biology?

    What is Cells Biology?

    Cells biology is the study of cell structure and function, and it revolves around the concept that the cell is the fundamental unit of life. Focusing on the cell permits a detailed understanding of the tissues and organisms that cells compose. Some organisms have only one cell, while others are organized into cooperative groups with huge numbers of cells. On the whole, cell biology focuses on the structure and function of a cell, from the most general properties shared by all cells, to the unique, highly intricate functions particular to specialized cells.

    Cells Defined: One of the basic tenets of biology is that all living things are composed of one or more cells. Some organisms consist of a single cell, while others have multiple cells organized into tissues, and tissues organized into organs. In many living things, organs function together as an organ system. However, even in these complex organisms, the basic biology revolves around the activities of the cell.

    Cells Biology

    The starting point for this discipline might be considered the 1830s. Though scientists had been using microscopes for centuries, they were not always sure what they were looking at. Robert Hooke’s initial observation in 1665 of plant-cell walls in slices of cork was followed shortly by Antonie van Leeuwenhoek’s first descriptions of live cells with visible moving parts. In the 1830s two scientists who were colleagues Schleiden, looking at plant cells, and Schwann, looking first at animal cells provided the first clearly stated definition of the cell. Their definition stated that all living creatures, both simple and complex, are made out of one or more cells, and the cell is the structural and functional unit of life a concept that became known as cell theory.

    As microscopes and staining techniques improved over the nineteenth and twentieth centuries, scientists were able to see more and more internal detail within cells. The microscopes used by van Leeuwenhoek probably magnified specimens a few hundredfold. Today high-powered electron microscopes can magnify specimens more than a million times and can reveal the shapes of organelles at the scale of a micrometer and below. With confocal microscopy a series of images can be combined, allowing researchers to generate detailed three-dimensional representations of cells. These improved imaging techniques have helped us better understand the wonderful complexity of cells and the structures they form.

    There are several main subfields within cell biology. One is the study of cell energy and the biochemical mechanisms that support cell metabolism. As cells are machines unto themselves, the focus on cell energy overlaps with the pursuit of questions of how energy first arose in original primordial cells, billions of years ago. Another subfield of cell biology concerns the genetics of the cell and its tight interconnection with the proteins controlling the release of genetic information from the nucleus to the cell cytoplasm. Yet another subfield focuses on the structure of cell components, known as subcellular compartments. Cutting across many biological disciplines is the additional subfield of cell biology, concerned with cell communication and signaling, concentrating on the messages that cells give to and receive from other cells and themselves. And finally, there is the subfield primarily concerned with the cell cycle, the rotation of phases beginning and ending with cell division and focused on different periods of growth and DNA replication. Many cell biologists dwell at the intersection of two or more of these subfields as our ability to analyze cells in more complex ways expands.

    In line with the continually increasing interdisciplinary study, the recent emergence of systems biology has affected many biological disciplines; it is a methodology that encourages the analysis of living systems within the context of other systems. In the field of cell biology, systems biology has enabled the asking and answering of more complex questions, such as the interrelationships of gene regulatory networks, evolutionary relationships between genomes, and the interactions between intracellular signaling networks. Ultimately, the broader a lens we take on our discoveries in cell biology, the more likely we can decipher the complexities of all living systems, large and small.

    One of the first scientists to observe cells was Englishman Robert Hooke. In the mid-1600s, Hooke examined a thin slice of cork through the newly developed microscope. The microscopic compartments in the cork impressed him and reminded him of rooms in a monastery, known as cells. He therefore referred to the units as cells. Later in that century, Anton Van Leeuwenhoek, a Dutch merchant, made further observations of plant, animal, and microorganism cells. In 1838, German botanist Matthias Schleiden proposed that all plants are composed of cells. A year later, his colleague, anatomist Theodor Schwann, concluded that all animals are also composed of cells. In 1858, biologist Rudolf Virchow proposed that all living things are made of cells and that all cells arise from preexisting cells. These premises have come down to us as the cell theory.

    Cells

    Movement Through the Plasma Membrane

    In order for the cell cytoplasm to communicate with the external environment, materials must be able to move through the plasma membrane. This movement occurs through several mechanisms.

    Diffusion: One method of movement through the membrane is diffusion. Diffusion is the movement of molecules from a region of higher concentration to one of lower concentration. This movement occurs because the molecules are constantly colliding with one another. The net movement of the molecules is away from the region of high concentration to the region of low concentration.

    Diffusion is a random movement of molecules down the pathway called the concentration gradient. Molecules are said to move down the concentration gradient because they move from a region of higher concentration to a region of lower concentration. A drop of dye placed in a beaker of water illustrates diffusion as the dye molecules spread out and color the water.

    Osmosis: Another method of movement across the membrane is osmosis. Osmosis is the movement of water from a region of higher concentration to one of lower concentration. Osmosis occurs across a membrane that is semipermeable. A semipermeable membrane lets only certain molecules pass through while keeping other molecules out. Osmosis is really a type of diffusion involving only water molecules.

    Facilitated diffusion: A third mechanism for movement across the plasma membrane is facilitated diffusion. Certain proteins in the membrane assist facilitated diffusion by permitting only certain molecules to pass across the membrane. The proteins encourage movement in the direction that diffusion would normally take place, from a region with a higher concentration of molecules to a region of lower concentration.

    Active transport: A fourth method for movement across the membrane is active transport. When active transport is taking place, a protein moves a certain material across the membrane from a region of lower concentration to a region of higher concentration. Because this movement is happening against the concentration gradient, the cell must expend energy that is usually derived from a substance called adenosine triphosphate, or ATP (see Chapter 4). An example of active transport occurs in human nerve cells. Here, sodium ions are constantly transported out of the cell into the external fluid bathing the cell, a region of high concentration of sodium. (This transport of sodium sets up the nerve cell for the impulse that will occur within it later.)

    Endocytosis and exocytosis: The final mechanism for movement across the plasma membrane into the cell is endocytosis, a process in which a small patch of plasma membrane encloses particles or tiny volumes of fluid that are at or near the cell surface. The membrane enclosure then sinks into the cytoplasm and pinches off from the membrane, forming a vesicle that moves into the cytoplasm. When the vesicle contains solid particulate matter, the process is called phagocytosis. When the vesicle contains droplets of fluid, the process is called pinocytosis. Along with the other mechanisms for transport across the plasma membrane, endocytosis ensures that the internal cellular environment will be able to exchange materials with the external environment and that the cell will continue to thrive and function. Exocytosis is the reverse of endocytosis, where internally produced substances are enclosed in vesicles and fuse with the cell membrane, releasing the contents to the exterior of the cell.

    The Structure of Prokaryote and Eukaryote Cells

    During the 1950s, scientists developed the concept that all organisms may be classified as prokaryotes or eukaryotes. The cells of all prokaryotes and eukaryotes possess two basic features: a plasma membrane, also called a cell membrane, and cytoplasm. However, the cells of prokaryotes are simpler than those of eukaryotes. For example, prokaryotic cells lack a nucleus, while eukaryotic cells have a nucleus. Prokaryotic cells lack internal cellular bodies (organelles), while eukaryotic cells possess them. Examples of prokaryotes are bacteria and archaea. Examples of eukaryotes are protists, fungi, plants, and animals (everything except prokaryotes).

    Plasma membrane: All prokaryote and eukaryote cells have plasma membranes. The plasma membrane (also known as the cell membrane) is the outermost cell surface, which separates the cell from the external environment. The plasma membrane is composed primarily of proteins and lipids, especially phospholipids. The lipids occur in two layers (a bilayer). Proteins embedded in the bilayer appear to float within the lipid, so the membrane is constantly in flux. The membrane is therefore referred to as a fluid mosaic structure. Within the fluid mosaic structure, proteins carry out most of the membrane’s functions.

    The “Movement through the Plasma Membrane” section later in this chapter describes the process by which materials pass between the interior and exterior of a cell.

    Cytoplasm and organelles: All prokaryote and eukaryote cells also have cytoplasm (or cytosol), a semiliquid substance that composes the volume of a cell. Essentially, cytoplasm is the gel-like material enclosed by the plasma membrane.

    Within the cytoplasm of eukaryote cells are a number of membrane-bound bodies called organelles (“little organs”) that provide a specialized function within the cell.

    One example of an organelle is the endoplasmic reticulum (ER). The ER is a series of membranes extending throughout the cytoplasm of eukaryotic cells. In some places, the ER is studded with submicroscopic bodies called ribosomes. This type of ER is called rough ER. In other places, there are no ribosomes. This type of ER is called smooth ER. The rough ER is the site of protein synthesis in a cell because it contains ribosomes; however, the smooth ER lacks ribosomes and is responsible for producing lipids. Within the ribosomes, amino acids are actually bound together to form proteins. Cisternae are spaces within the folds of the ER membranes.

    Another organelle is the Golgi apparatus (also called Golgi body). The Golgi apparatus is a series of flattened sacs, usually curled at the edges. In the Golgi body, the cell’s proteins and lipids are processed and packaged before being sent to their final destination. To accomplish this function, the outermost sac of the Golgi body often bulges and breaks away to form drop like vesicles known as secretory vesicles.

    An organelle called the lysosome (see Figure) is derived from the Golgi body. It is a drop like sac of enzymes in the cytoplasm. These enzymes are used for digestion within the cell. They break down particles of food taken into the cell and make the products available for use; they also help break down old cell organelles. Enzymes are also contained in a cytoplasmic body called the peroxisome.

    Diagram of an Animal Cells Biology
    Diagram of an Animal Cells Biology

    Figure The components of an idealized eukaryotic cell. The diagram shows the relative sizes and locations of the cell parts.

    The organelle that releases quantities of energy to form adenosine triphosphate (ATP) is the mitochondrion (the plural form is mitochondria). Because mitochondria are involved in energy release and storage, they are called the “powerhouses of the cells.”

    Green plant cells, for example, contain organelles known as chloroplasts, which function in the process of photosynthesis. Within chloroplasts, energy from the sun is absorbed and transformed into the energy of carbohydrate molecules. Plant cells specialized for photosynthesis contain large numbers of chloroplasts, which are green because the chlorophyll pigments within the chloroplasts are green. Leaves of a plant contain numerous chloroplasts. Plant cells not specializing in photosynthesis (for example, root cells) are not green.

    An organelle found in mature plant cells is a large, fluid-filled central vacuole. The vacuole may occupy more than 75 percent of the plant cell. In the vacuole, the plant stores nutrients, as well as toxic wastes. Pressure within the growing vacuole may cause the cell to swell.

    The cytoskeleton is an interconnected system of fibers, threads, and interwoven molecules that give structure to the cell. The main components of the cytoskeleton are microtubules, microfilaments, and intermediate filaments. All are assembled from subunits of protein.

    The centriole organelle is a cylinder like structure that occurs in pairs. Centrioles function in cell division.

    Many cells have specialized cytoskeletal structures called flagella and cilia. Flagella are long, hair like organelles that extend from the cell, permitting it to move. In prokaryotic cells, such as bacteria, the flagella rotate like the propeller of a motorboat. In eukaryotic cells, such as certain protozoa and sperm cells, the flagella whip about and propel the cell. Cilia are shorter and more numerous than flagella. In moving cells, the cilia wave in unison and move the cell forward. Paramecium is a well-known ciliated protozoan. Cilia are also found on the surface of several types of cells, such as those that line the human respiratory tract.

    Nucleus: Prokaryotic cells lack a nucleus; the word prokaryotic means “primitive nucleus.” Eukaryotic cells, on the other hand, have a distinct nucleus.

    The nucleus of eukaryotic cells is composed primarily of protein and deoxyribonucleic acid, or DNA. The DNA is tightly wound around special proteins called histones; the mixture of DNA and histone proteins is called chromatin. The chromatin is folded even further into distinct threads called chromosomes. Functional segments of the chromosomes are referred to as genes. Approximately 21,000 genes are located in the nucleus of all human cells.

    The nuclear envelope, an outer membrane, surrounds the nucleus of a eukaryotic cell. The nuclear envelope is a double membrane, consisting of two lipid layers (similar to the plasma membrane). Pores in the nuclear envelope allow the internal nuclear environment to communicate with the external nuclear environment.

    Within the nucleus are two or more dense organelles referred to as nucleoli (the singular form is nucleolus). In nucleoli, submicroscopic particles known as ribosomes are assembled before their passage out of the nucleus into the cytoplasm.

    Although prokaryotic cells have no nucleus, they do have DNA. The DNA exists freely in the cytoplasm as a closed loop. It has no protein to support it and no membrane covering it. A bacterium typically has a single looped chromosome.

    Cell Wall

    Many kinds of prokaryotes and eukaryotes contain a structure outside the cell membrane called the cell wall. With only a few exceptions, all prokaryotes have thick, rigid cell walls that give them their shape. Among the eukaryotes, some protists, and all fungi and plants, have cell walls. Cell walls are not identical in these organisms, however. In fungi, the cell wall contains a polysaccharide called chitin. Plant cells, in contrast, have no chitin; their cell walls are composed exclusively of the polysaccharide cellulose.

    Cell walls provide support and help cells resist mechanical pressures, but they are not solid, so materials are able to pass through rather easily. Cell walls are not selective devices, as plasma membranes are.

  • What is Cells?

    What is Cells?

    The cell (from Latin cella, meaning “small room”) is the basic structural, functional, and biological unit of all known living organisms. A cell is the smallest unit of life that can replicate independently, and cells are often called the “building blocks of life”. The study of cells is called cell biology.

    Cells

    Cells consist of cytoplasm enclosed within a membrane, which contains many biomolecules such as proteins and nucleic acids. Organisms can be classified as unicellular (consisting of a single cell; including bacteria) or multicellular (including plants and animals). While the number of cells in plants and animals varies from species to species, humans contain more than 10 trillion (1012) cell. Most plant and animal cells are visible only under a microscope, with dimensions between 1 and 100 micrometers.

    The cell was discovered by Robert Hooke in 1665, who named the biological unit for its resemblance to cell inhabited by Christian monks in a monastery. Cell theory, first developed in 1839 by Matthias Jakob Schleiden and Theodor Schwann, states that all organisms are composed of one or more cell, that cell are the fundamental unit of structure and function in all living organisms, that all cell come from preexisting cell, and that all cell contain the hereditary information necessary for regulating cell functions and for transmitting information to the next generation of cells. Cells emerged on Earth at least 3.5 billion years ago.

    Types of Cells

    Eukaryote and Prokaryote Cells
    Eukaryote and Prokaryote

    Prokaryote Cells

    Prokaryotic cells were the first form of life on Earth, characterized by having vital biological processes including cell signaling and being self-sustaining. They are simpler and smaller than eukaryotic cells and lack membrane-bound organelles such as the nucleus. Prokaryotes include two of the domains of life, bacteria, and archaea. The DNA of a prokaryotic cell consists of a single chromosome that is in direct contact with the cytoplasm. The nuclear region in the cytoplasm is called the nucleoid. Most prokaryotes are the smallest of all organisms ranging from 0.5 to 2.0 µm in diameter.

    A prokaryotic cell has three architectural regions:

    I. Enclosing the cell is the cell envelope generally consisting of a plasma membrane covered by a cell wall which, for some bacteria, may be further covered by a third layer called a capsule. Though most prokaryotes have both a cell membrane and a cell wall, there are exceptions such as Mycoplasma (bacteria) and Thermo-plasma (archaea) which only possess the cell membrane layer. The envelope gives rigidity to the cell and separates the interior of the cell from its environment, serving as a protective filter. The cell wall consists of peptidoglycan in bacteria and acts as an additional barrier against exterior forces. It also prevents the cell from expanding and bursting (cytolysis) from osmotic pressure due to a hypotonic environment. Some eukaryotic cells (plant cells and fungal cells) also have a cell wall.

    II. Inside the cell is the cytoplasmic region that contains the genome (DNA), ribosomes and various sorts of inclusions. The genetic material is freely found in the cytoplasm. Prokaryotes can carry extrachromosomal DNA elements called plasmids, which are usually circular. Linear bacterial plasmids have been identified in several species of spirochete bacteria, including members of the genus Borrelia notably Borrelia burgdorferi, which causes Lyme disease. Though not forming a nucleus, the DNA is condensed in a nucleoid. Plasmids encode additional genes, such as antibiotic resistance genes.

    III. On the outside, flagella and pili project from the cell’s surface. These are structures (not present in all prokaryotes) made of proteins that facilitate movement and communication between cells.

    The Structure of Prokaryote and Eukaryote Cells
    The Structure of Prokaryote and Eukaryote Cells

    Eukaryote Cells

    Plants, animals, fungi, slime moulds, protozoa, and algae are all eukaryotic. These cells are about fifteen times wider than a typical prokaryote and can be as much as a thousand times greater in volume. The main distinguishing feature of eukaryotes as compared to prokaryotes is compartmentalization: the presence of membrane-bound organelles (compartments) in which specific metabolic activities take place. Most important among these is a cell nucleus, an organelle that houses the cell’s DNA. This nucleus gives the eukaryote its name, which means “true kernel (nucleus)”. Other differences include:

    I. The plasma membrane resembles that of prokaryotes in function, with minor differences in the setup. Cell walls may or may not be present.

    II. The eukaryotic DNA is organized in one or more linear molecules, called chromosomes, which are associated with histone proteins. All chromosomal DNA is stored in the cell nucleus, separated from the cytoplasm by a membrane. Some eukaryotic organelles such as mitochondria also contain some DNA.

    III. Many eukaryotic cell are ciliated with primary cilia. Primary cilia play important roles in chemosensation, mechanosensation, and thermosensation. Cilia may thus be “viewed as a sensory cellular antenna that coordinates a large number of cellular signaling pathways, sometimes coupling the signaling to ciliary motility or alternatively to cell division and differentiation.”

    IV. Motile cells of eukaryotes can move using motile cilia or flagella. Motile cells are absent in conifers and flowering plants. Eukaryotic flagella are less complex than those of prokaryotes.

  • What is Biology?

    What is Biology?

    The study of living organisms, divided into many specialized fields that cover their morphology, physiology, anatomy, behavior, origin, and distribution; The plants and animals of a particular area, and the physiology, behavior, and other qualities of a particular organism or class of organisms.

    Biology is a natural science concerned with the study of life and living organisms, including their structure, function, growth, evolution, distribution, identification and taxonomy. Modern biology is a vast and eclectic field, composed of many branches and sub disciplines. However, despite the broad scope of biology, there are certain general and unifying concepts within it that govern all study and research, consolidating it into single, coherent field. In general, biology recognizes the cell as the basic unit of life, genes as the basic unit of heredity, and evolution as the engine that propels the synthesis and creation of new species. It is also understood today that all the organisms survive by consuming and transforming energy and by regulating their internal environment to maintain a stable and vital condition known as homeostasis.

    History of Biology:

    The term biology is derived from the Greek word βίος, bios, “life” and the suffix -λογία, -logia, “study of.” The Latin-language form of the term first appeared in 1736 when Swedish scientist Carl Linnaeus (Carl von Linné) used biologi in his Bibliotheca botanica. It was used again in 1766 in a work entitled Philosophiae Naturalis sive physicae: tomus III, Continens Geologian, biologian, phytologian generalis, by Michael Christoph Hanov, a disciple of Christian Wolff. The first German use, Biologie, was in a 1771 translation of Linnaeus’ work. In 1797, Theodor Georg August Roose used the term in the preface of a book, Grundzüge der Lehre van der Lebenskraft. Karl Friedrich Burdach used the term in 1800 in a more restricted sense of the study of human beings from a morphological, physiological and psychological perspective (Propädeutik zum Studien der GE Samm ten Heilkunst). The term came into its modern usage with the six-volume treatise Biologie, oder Philosophie der lebenden Natur (1802–22) by Gottfried Reinhold Treviranus, who announced; The objects of our research will be the different forms and manifestations of life, the conditions and laws under which these phenomena occur, and the causes through which they have been effected. The science that concerns itself with these objects we will indicate by the name biology [Biologie] or the doctrine of life [Lebenslehre].

    Biology is the study of living things. It encompasses the cellular basis of living things, the energy metabolism that underlies the activities of life, and the genetic basis for inheritance in organisms. Biology also includes the study of evolutionary relationships among organisms and the diversity of life on Earth. It considers the biology of microorganisms, plants, and animals, for example, and it brings together the structural and functional relationships that underlie their day-to-day activities. Biology draws on the sciences of chemistry and physics for its foundations and applies the laws of these disciplines to living things.

    Biology living world scale
    Biology – Living World Scale

    Many sub disciplines and special areas of biology exist, which can be conveniently divided into practical and theoretical categories. Types of practical biology include plant breeding, wildlife management, medical science, and crop production. Theoretical biology encompasses such disciplines as physiology (the study of the function of living things), biochemistry (the study of the chemistry of organisms), taxonomy (classification), ecology (the study of populations and their interactions with each other and their environments), and microbiology (the study of microscopic organisms).

    Their fascination with biology has a long history. Even early humans had to study the animals that they hunted and know where to find the plants that they gathered for food. The invention of agriculture was the first great advance of human civilization. Medicine has been important to us from earliest history as well. The earliest known medical texts are from China (2500 B.C.), Mesopotamia (2112 B.C.), and Egypt (1800 B.C.).

    In classical times, Aristotle is often considered to be the first to practice scientific zoology. He is known to have performed extensive studies of marine life and plants. His student, Theophrastus, wrote one of the West’s earliest known botanical texts in 300 B.C. on the structure, life cycle and uses of plants. The Roman physician Galen used his experience in patching up gladiators for the arena to write texts on surgical procedures in A.D. 158.

    During the Renaissance, Leonardo da Vinci risked censure by participating in human dissection and making detailed anatomical drawings that are still considered among the most beautiful ever made. Invention of the printing press and the ability to reproduce woodcut illustrations meant that information was much easier to record and disseminate. One of the first illustrated biology books is a botanical text written by German botanist Leonhard Fuchs in 1542. Binomial classification was inaugurated by Carolus Linnaeus in 1735, using Latin names to group species according to their characteristics.

    Microscopes opened up new worlds for scientists. In 1665, Robert Hooke, used a simple compound microscope to examine a thin sliver of cork. He observed that the plant tissue consisted of rectangular units that reminded him of the tiny rooms used by monks. He called these units “cells.” In 1676, Anton von Leeuwenhoek published the first drawings of living single celled organisms. Theodore Schwann added the information that animal tissue is also composed of cells in 1839.

    During the Victorian era, and throughout the 19th century, “Natural Science” became something of a mania. Thousands of new species were discovered and described by intrepid adventurers and by backyard botanists and entomologists alike. In 1812, Georges Cuvier described fossils and hypothesized that Earth had undergone “successive bouts of Creation and destruction” over long periods of time. On Nov. 24, 1859, Charles Darwin published “On the Origin of Species,” the text that forever changed the world by showing that all living things are interrelated and that species were not separately created but arise from ancestral forms that are changed and shaped by adaptation to their environment.

    While much of the world’s attention was captured by biology questions at the macroscopic organism level, a quiet monk was investigating how living things pass traits from one generation to the next. Gregor Mendel is now known as the father of genetics although is papers on inheritance, published in 1866, went largely unnoticed at the time. His work was rediscovered in 1900 and further understanding of inheritance rapidly followed.

    The 20th and 21st centuries may be known to future generations as the beginning of the “Biological Revolution.” Beginning with Watson and Crick explaining the structure and function of DNA in 1953, all fields of biology have expanded exponentially and touch every aspect of our lives. Medicine will be changed by development of therapies tailored to a patient’s genetic blueprint or by combining biology and technology with brain-controlled prosthetics. Economies hinge on the proper management of ecological resources, balancing human needs with conservation. We may discover ways to save our oceans while using them to produce enough food to feed the nations. We may “grow” batteries from bacteria or light buildings with bioluminescent fungi. The possibilities are endless; biology is just coming into its own.

    Characteristics of Living Things:

    Defining a living thing is a difficult proposition, as is defining “life”—that property possessed by living things. However, a living thing possesses certain properties that help define what life is.

    Biology Human Lifecycle
    Biology Human Life-cycle

    Complex organization: Living things have a level of complexity and organization not found in lifeless objects. At its most fundamental level, a living thing is composed of one or more cells. These units, generally too small to be seen with the naked eye, are organized into tissues. A tissue is a series of cells that accomplish a shared function. Tissues, in turn, form organs, such as the stomach and kidney. A number of organs working together compose an organ system. An organism is a complex series of various organ systems.

    Metabolism: Living things exhibit a rapid turnover of chemical materials, which is referred to as metabolism. Metabolism involves exchanges of chemical matter with the external environment and extensive transformations of organic matter within the cells of a living organism. Metabolism generally involves the release or use of chemical energy. Nonliving things do not display metabolism.

    Responsiveness: All living things are able to respond to stimuli in the external environment. For example, living things respond to changes in light, heat, sound, and chemical and mechanical contact. To detect stimuli, organisms have means for receiving information, such as eyes, ears, and taste buds.

    To respond effectively to changes in the environment, an organism must coordinate its responses. A system of nerves and a number of chemical regulators called hormones coordinate activities within an organism. The organism responds to the stimuli by means of a number of effectors, such as muscles and glands. Energy is generally used in the process.

    Organisms change their behavior in response to changes in the surrounding environment. For example, an organism may move in response to its environment. Responses such as this occur in definite patterns and make up the behavior of an organism. The behavior is active, not passive; an animal responding to a stimulus is different from a stone rolling down a hill. Living things display responsiveness; nonliving things do not.

    Growth: Growth requires an organism to take in material from the environment and organize the material into its own structures. To accomplish growth, an organism expends some of the energy it acquires during metabolism. An organism has a pattern for accomplishing the building of growth structures.

    During growth, a living organism transforms material that is unlike itself into materials that are like it. A person, for example, digests a meal of meat and vegetables and transforms the chemical material into more of himself or herself. A nonliving organism does not display this characteristic.

    Reproduction: A living thing has the ability to produce copies of itself by the process known as reproduction. These copies are made while the organism is still living. Among plants and simple animals, reproduction is often an extension of the growth process. More complex organisms engage in a type of reproduction called sexual reproduction, in which two parents contribute to the formation of a new individual. During this process, a new combination of traits can be produced.

    Asexual reproduction involves only one parent, and the resulting cells are generally identical to the parent cell. For example, bacteria grow and quickly reach maturity, after which they split into two organisms by a process of asexual reproduction called binary fission.

    Evolution: Living organisms have the ability to adapt to their environment through the process of evolution. During evolution, changes occur in populations, and the organisms in the population become better able to metabolize, respond, and reproduce. They develop abilities to cope with their environment that their ancestors did not have.

    Evolution also results in a greater variety of organisms than existed in previous eras. This proliferation of populations of organisms is unique to living things.

    Ecology: The environment influences the living things that it surrounds. Ecology is the study of relationships between organisms and their relationships with their environment. Both biotic factors (living things) and abiotic factors (non-living things) can alter the environment. Rain and sunlight are non-living components, for example, that greatly influence the environment. Living things may migrate or hibernate if the environment becomes difficult to live in.